Research

orcid-code_ashwani  

Broad Areas:
Skeletal Muscle Metabolism, Protein Biochemistry and Cell Signaling.

Prime interest of our research group is focused on the identification of natural bioactive compounds which can counteract the inflammatory molecules and reactive oxygen and nitrogen species, the key culprits for one of threatening clinical setting i.e. skeletal muscle atrophy. This setting gets triggered during aging and in variety of chronic disease states including diabetes, heart failure, cancer, AIDS etc and become the prominent cause of morbidity and mortality. Aim is also to reestablish the confidence, scientific credential and authentication level of the Ayurveda.

Research projects
Oct 2000 – Feb 2007
• Study of Dipeptidylaminopeptidases/ cathepsins/ xylanase enzymes by using different sources i.e. plants; microbial and animal brain for the purpose of its purification, enzyme kinetics and inhibition studies.
• Inhibition kinetics with some anti-tuberculous, anti-inflammatory and anti-leprotic drugs on cathepsins.
• Immobilization of DPP IV and its application in Bio-transformation” (UGC funded Minor Research Project, Delhi, India, 2006-2007). Principal Investigator

March 2007-August 2009
• Specific adrenergic receptors and PKC/ERKs isoforms in cerebral arterial contraction and their role in cerebral blood flow (NIH funded, USA – During Postdoc Fellowship)
• Role of cytokines in Skeletal Muscle Atrophy
Tumor Necrosis Factor-related Weak Inducer of Apoptosis is a potent muscle-wasting cytokine which induces atrophy in cultured skeletal muscle cells. (NIH funded, USA- During Postdoc Fellowship)

July 2012 – till date
• Effect of S-allyl cysteine on inflammatory and oxidative response in skeletal muscle metabolism (Funded by UGC, New Delhi, India – Rs 15.08 lacs – 2012 – 2015) – Principal Investigator
• Effect of Cinnamaldehyde in Skeletal Muscle Atrophy (Funded by Indian Council of Medical Research [ICMR], Delhi, India – Rs 31 lacs, 2015 – 2018) – Principal Investigator
• Strengthening of antioxidant defense system of human lymphocytes using Holy Basil (Ocimum sanctum) for prevention and management of chronic diseases prevailing in Haryana (Funded by Haryana-DST, India – Rs 10 lacs – 2015 – 2018) – Co-Principal Investigator

Besides these we are also dealing with medicinal plants including Saraca asoca and Tinospora cordiofolia which are being used traditionally in Indian medical system for the treatment for various diseases including microbial infections, leucorrhoea, menorrhagia, cancer, diabetes etc. By using the extracts of these plants, we establish their proteomics and metablomic profiles and also investigate their anti-inflammatory and anti-oxidative mechanism of action with the help of targeted and non-targeted LC-QTOF-MS and 2-D gel electrophoresis in C2C12 cell line based atrophic models.

In the present studies, we are using latest cutting edge technology including Western blot, EMSA assay, PCR, qRT-PCR, Fluorescent Microscopy, Immunoprecipitation, GST-, His-pull down assay etc. Along with these we are also using traditional techniques i.e. column chromatography (Gel filtration, Anion, Cation and Affinity) and Native Gel electrophoresis.

Awards and Fellowship
• University Research Fellowship (March 2000 – March 2003) – awarded by Kurukshetra University, Haryana, India.
• Raman Fellowship (by UGC) – To work in a Project entitle “Dicer and its role in skeletal muscle myogenesis” with Dr Atsushi Asakura at Stem Cell Institute, University of Minnesota, Minneapolis – USA (From Jan 2014 to June 2014).
• Honoured with Prof. M. L. Khorana Memorial Prize (Best Paper Award) for the year 2013 by the Indian Pharmaceutical Association, in the field of Pharmacology & Clinical Pharmacy (5th June 2015 at Hotel Trident, C-56, G Block, Bandra Kurla Complex, Mumbai).

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Reviewer of Reputed Journals
• Plos One (Since 2015)
• Scientific Reports (Since 2016)
• Molecular and Cellular Endocrinology (Since 2016)
• Pharmacognosy (Since 2015)
• Chemical Biology Letters (Since 2016)
• Cellular and Molecular Sciences (Since 2016)