Biochem-Biotech students

Enzyme Kinetics

Paper I

a. Explain the significance of Km and Kcat/Km. 5
b. Write the name of various factors which affect the enzyme activity. Write in detail about the affect of substrate concentration. 5
c. Explain sequential mechanism use by enzyme in bi-substrate reaction. 5
d. What is enzyme inhibition? Explain with example how Michaelis –Menten equation gets affected by competitive inhibitors. Also draw their Lineweaver-Burk plot. 15

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Write name of specific amino acids which are catalyzing the reaction of chymotrypsin and carboxypeptidase 5

Key:
Chymotrypsin: Asp(102), His(57), Ser(195): Carboxypeptidase –Metalloenzyme (Zn2+) Glu(270)